WebConsidering all combinations, we detected a marked gain of interaction at loci where H3K27ac was gained in the CTCF +/− cells (Fig. 6I). These results indicate that the … WebNov 3, 2011 · Here we provide the first evidence that a DNA-binding protein, CCCTC-binding factor (CTCF), can promote inclusion of weak upstream exons by mediating local RNA polymerase II pausing both in a mammalian model system for alternative splicing, CD45, and genome-wide.

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http://www.smc3.com/ WebJul 2, 2024 · The H3K27ac HiChIP data are used to map chromatin loops associated with active promoters and enhancers [ 51, 52 ], and Ocean-C, a recently developed antibody-free chromosome conformation capture technique, combines FAIRE-Seq with Hi-C to map hubs of open chromatin interactions [ 53 ]. iot for museum artifact management system

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WebWe performed HiC, chromatin conformation capture (Capture-C), single-nucleus multiomics (RNA-seq+ATAC-seq), bulk ATAC-seq, and ChIP-seq for H3K27Ac and CTCF in NPCs and neurons differentiated from human iPSC cultures. WebCTCF – Identifying actual chromatin loops. Meaning, CTCF-cohesin mediated looping. H3K27ac or H3K4me3 – means identifying regions that interact with the enhancer or active promotor marker respectively. These interactions do not necessarily reflect the canonical loop formation but could reflect short range folding. WebJan 18, 2024 · Looking specifically at H3K27Ac (a histone modification demarcating active promoters and enhancers [ 13 ]) HT-ChIPmentation and ChIPmentation samples generated in parallel from high cell-numbers (50–150 k cells), both methods generated high-quality data that is comparable in regard to: concordance of library profiles (Fig. 1 b); … onushilonedu.com