WebJan 1, 2011 · Hydrophobic interaction chromatography (HIC) is a commonly used technique that exploits these hydrophobic features of proteins as a basis for their separation even in complex biological... Web2 71712900 AH 1 Abstract Phenyl Sepharose™ High Performance and Butyl Sepharose High Performance are members of the Cytiva range of hydrophobic interaction chromatography (HIC) resins for intermediate purification and polishing of …

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WebCientistas identificam molécula que inibe proteína ligada ao crescimento do câncer. Grupo composto por cientistas do Brasil e da Alemanha tiveram um avanço… WebFigure 3 is an overview of Cytiva Capto™ HIC resins. Information about Capto™ Butyl ImpRes and Capto™ Phenyl ImpRes resins can be found in data file CY13700. 3 Fig 4. Relative hydrophobic scale of various resins, based on retention of ribonuclease A, lysozyme, and α-Chymotrypsin. Can change with running dynamics 365 hr dataverse

Column Packing and Preparation for Hydrophobic Interaction …

Hydrophobic interaction chromatography (HIC) separates proteins according to differences in their surface hydrophobicity. HIC utilizes a reversible interaction between the proteins and the hydrophobic ligand of a HIC resin. The interaction between hydrophobic proteins and a HIC resin is greatly … See more Proteins with different degrees of surface hydrophobicity can be separated using hydrophobic interaction chromatography. The proteins are … See more Hydrophobic interaction chromatography can be used for capture, intermediate purification, or polishing steps. Samples should be in a high salt concentration to promote hydrophobic … See more Reversed phase chromatography also separates proteins and peptides on the basis of hydrophobicity. Compared with a HIC resin, the surface/ligands of an RPC resin are usually more hydrophobic. This leads to stronger … See more WebCytiva™ Description General description Useful for the intermediate stages of protein purification, Capto Butyl HIC resin combines high capacity, high flow rate, and low back pressure to reduce process cycle times. … WebHIC is commonly used as a polishing step in mAb purification processes. It can be used to remove mAb product-related impurities and process contaminants, such as host cell proteins (HCPs), leached protein A, and endogenous viruses, in both analytical and preparatory scale applications. dynamics 365 http error 500