Fathead minnow fhm cells
WebAug 1, 1987 · Overall, the FHM cells were more sensitive than were the BF-2 cells. However, there was a better correlation between the in vitro cytotoxicity data for the BF-2 cell culture and LC 50 data for bluegill sunfish than between similar data for the FHM cell line and fathead minnows. WebABSTRACT: Invasion of Edwardsiella ictaluri into cultured mammalian, fish and enzymatically harvested catfish enteric epithelial cells is described. Gentamicin survival assays were used to demonstrate the ability of this catfish pathogen to invade IEC-6 (origin: rat small intestinal epithelium), Henle 407 (origin: human embryonic intestinal …
Fathead minnow fhm cells
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WebMar 11, 2024 · In fish cells, there are a handful of carefully controlled experiments with convincing evidence of gene knockdown driven by shRNA-produced by a plasmid. Xie et al. provided convincing data on the fathead minnow (FHM) cell line at 29°C by using co-transfection experiment with a lacZ- and corresponding shRNA-expressing plasmids. … WebIncreased cells (spermatogonia) in fathead minnow testis: (a) Testis from adult male FHM negative control. (b) Spermatogonia dominate the germinal epithelium in this testis from adult male FHM exposed to 10 nM 17ß -estradiol for 10 days. Other diagnoses for this section include “decreased cells, spermatocytes” and “decreased cells ...
http://www.taallakehatchery.com/fathead/ WebFathead minnows are found in every drainage in Minnesota. It is the most common species of minnow in the state. They live in many kinds of lakes and streams, but are especially …
WebFeb 14, 2024 · The Fathead Minnow is the most affordable and durable of the forage species. We are able to stock them in all but the hottest temperatures. It is a small prolific … WebThe virus can be propagated to a limited extent in fathead minnow Pimephales promelas (FHM), Epithelioma papulosum cyprinid (EPC), and fin tissue of orange-spotted grouper Epinephelus coioides (GF-1) cell lines (Kongtorp and Taksdal, 2009; Mikalsen et al., 2012).
WebJul 3, 2024 · The cell line designated FHM, originating from tissue of the northern fathead minnow (Pimephales promelas) (Gravell and Malsberger 2010). Until lately, FHM cells are able to evaluate the oxicity of methyl mercury (Devlin and Clary 1998 ), the LSER molecular parameters for acute toxicity prediction (Lyakurwa et al. 2014 ), and the influence of ...
WebThe resulting superna-tant was stored overnight at 4 °C and inoculated onto epithelioma papulosum cyprini (EPC) and fathead minnow (FHM) cell lines cultured in min-imum essential medium (Gibco) supplemented with 10% foetal bovine serum (FBS) (Fryer & Lannan 1994). Cell cultures were incubated at 20 °C; when a cytopathic effect (CPE) … moriarty full nameWebIn fathead minnows the choroid body, surrounding the optic nerve, is a well developed and intricate array of arterioles and capillaries forming a reté mirabile. This organ is designed to satisfy the high oxygen demands of the poorly vascularized retina. The iris, a continuation of the choroid body, projects in a thin layer over the anterior ... moriarty funeral homeWebSep 21, 2024 · In order to determine the subcellular localization and study the preliminary function of SOD from N. seriolae (NsSOD), gene cloning, secreted protein identification, subcellular localization in fish cells, and apoptosis detection of NsSOD were carried out in this study. Subcellular localization research revealed that NsSOD-GFP fusion proteins ... moriarty from sherlock holmes