Perk antibody cst
WebA test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Staining Protocol: We recommend using Protocol C: Two-step protocol: Fixation/Methanol. WebPERK Rabbit pAb (A18196) Publications (12) Datasheet SDS. Tested applications: WB IF/ICC Reactivity: Human, Mouse, Rat. Western blot analysis of extracts of Mouse thymus, using …
Perk antibody cst
Did you know?
WebApr 18, 2011 · The epidermal growth factor receptor (EGFR) is overexpressed or mutated in glioma. Recently, a series of missense mutations in the extracellular domain (ECD) of EGFR were reported in glioma patients. Some of these mutations clustered within a cysteine-rich region of the EGFR targeted by the therapeutic antibody mAb806. This region is only … WebWestern blot analysis of extracts of Mouse thymus, using PERK antibody (A18196) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
WebThe following primary antibodies were used: pERK1/2 Thr202/Tyr204, # 9101; ERK1/2, # 9102; pSTAT3 Tyr705, # 9145; STAT3, # 9132; ATGL, # 2138; pHSL Ser563, # 4139; HSL, # 4107; Perilipin -1 December 31, 2024 Pim P. van Krieken, Julian Roos, Pamela Fischer-Posovszky, Stephan Wueest, Daniel Konrad WebWestern blot band for Erk and phopho (p)-Erk I want to know Erk activity in my animal model - sepsis. I isolated it from liver, homogenized it, separated it by SDS-PAGE, and detected it, but I...
Protein kinase-like endoplasmic reticulum kinase (PERK) is an eIF2α kinase and transmembrane protein resident in the endoplasmic reticulum (ER) membrane that couples ER stress signals to translation inhibition (1-3). ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation. WebPERK Antibody (B-5) is a mouse monoclonal IgG 1 κ PERK antibody, cited in 89 publications, provided at 200 µg/ml raised against amino acids 21-320 mapping near the N-terminus of …
WebPERK ELISA Kits Listed are ELISA Kits for the detection of PERK, an alias name of eukaryotic translation initiation factor 2 alpha kinase 3. The human protein, encoded by the gene EIF2AK3, is 1116 amino acid residues long and has a mass of 125,216 daltons.
WebOct 19, 2024 · The epidermal growth factor receptor (EGFR) is a well-characterized cluster-based receptor tyrosine kinase that initiates signaling cascades promoting cell growth, proliferation, and differentiation and is therefore implicated in a variety of human cancers [9–12].Notably, in previous work [3, 13], we have gained mechanistic insight into EGFR … pre ban firearms for saleWebFeb 5, 2024 · ERK 1/2 Antibody (C-9) is a mouse monoclonal IgG 2a κ ERK 1/2 antibody, cited in 460 publications, provided at 200 µg/ml raised against amino acids 101-172 … scooter mcquade\u0027s portland orWebApr 13, 2024 · The Unfolded protein response (UPR), triggered by stress in the endoplasmic reticulum (ER), is a key driver of neurodegenerative diseases. GM2 gangliosidosis, which includes Tay-Sachs and Sandhoff disease, is caused by an accumulation of GM2, mainly in the brain, that leads to progressive neurodegeneration. Previously, we demonstrated in a … pre ban bushmaster